SENSITIVITY AND FIDELITY OF DNA MICROARRAY IMPROVED WITH INTEGRATION OF AMPLIFIED DIFFERENTIAL GENE EXPRESSION (ADGE)

Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)

Abstract Background The ADGE technique is a method designed to magnify the ratios of gene expression before detection.It improves the detection sensitivity to small change of gene expression and requires small amount of starting material.However, the throughput of ADGE is low.We integrated ADGE with DNA microarray (ADGE microarray) and compared it

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Genetics and immunity of Anopheles response to the entomopathogenic fungus Metarhizium anisopliae overlap with immunity to Plasmodium

Abstract Entomopathogenic fungi have been explored as a potential biopesticide to counteract the insecticide resistance issue in mosquitoes.However, little is known about the possibility that genetic resistance to fungal biopesticides could evolve in mosquito u11-200ps populations.Here, we detected an important genetic component underlying Anophele

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An analytical comparison of three immunoassay platforms for subpicomolar detection of protein biomarker GAD65.

A disproportional increase of circulating GAD65 within hours from an intraportal islet allotransplantation has been validated as biomarker of beta cell loss and poor functional outcome.More sensitive assays are, however, needed to allow detection of episodes of subtle beta cell loss during late-stage graft rejection or in the peri-onset period of t

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